反相流动注射化学发光法测定盐酸小檗碱

在酸性条件下,盐酸小檗碱对联吡啶钌(Ⅱ)-铈(Ⅳ)反应体系的化学发光有显著的增强作用,据此建立了反相流动注射化学发光法测定盐酸小檗碱的新方法.对影响流动注射化学发光的各因素进行了实验研究,优化了反应条件和各项测定参数.结果表明,优化条件下,在质量浓度为1.0×10-7~1.5×10-5g/mL的范围内,相对发光强度与盐酸小檗碱的浓度成对数线性关系,检出限(3σ)为3.6×10-8g/mL,对浓度为1.0×10-6g/mL的盐酸小檗碱进行11次平行测定,相对标准偏差(RSD)为2.3%.本方法用于复方黄连素片剂中盐酸小檗碱含量的测定,具有良好的选择性,标准加入法的回收率在97.8%~101.5%之间.分析效率为140/h.     

盐酸-结晶紫底液中碲(Ⅳ)的极谱波性质之研究

本文研究了Te(Ⅳ)在盐酸-结晶紫底液中的极谱波特性,结果表明:当Te(Ⅳ)浓度小于80ppb时,为氢催化波;而当其浓度约高于80ppb时,其还原波还叠加了Te(0)与结晶紫在汞电极表面上生成的电活性配合物之吸附波.     

The involvement of the renin-angiotensin system in the regulation of cell proliferation in the rat endometrium

Oestrogens are known to enhance angiotensin biosynthesis by increasing the elaboration of its precursor, angiotensinogen. On the other hand, we found that inhibition of angiotensin-converting enzyme (ACE) suppressed the proliferative response of the rat anterior pituitary gland to oestrogens. To answer the question whether the angiotensin system is involved in the control of the cell proliferation of the uterine epithelium, the effects of an ACE inhibitor, enalapril maleate, and of angiotensins II and IV, alone or together with losartan, an antagonist of angiotensin receptor type 1 (AT1), on endometrial epithelial cell proliferation have been studied. The experiments were performed on ovariectomized female Wistar rats. In the first experiment the animals were injected with a single dose of oestradiol benzoate or received an injection of solvent only. Half of the oestrogen-treated rats were injected additionally with enalapril maleate (EN, twice daily). The incorporation of bromodeoxyuridine (BrDU) into endometrial cell nuclei was used as an index of cell proliferation. It was found that oestradiol alone dramatically increased the BrDU labelling index (LI) of endometrial cell nuclei, and this effect was partially blocked by the simultaneous treatment with EN. In the second experiment, the animals were injected intraperitoneally with angiotensin II (AII), angiotensin IV (AIV) or saline, alone or together with losartan. It was found that AIV induced an increase in the LI in uterine epithelium, and this effect was not blocked by the simultaneous treatment with losartan. The increase in LI in uterine epithelium was also observed in the rats treated with AII and with losartan. These findings suggest an involvement of angiotensin IV in the control of uterine epithelium cell proliferation. Received 12 October 1998; received after revision 6 January 1999; accepted 2 February 1999     

中药复方“通脉口服液”治疗慢性肾炎气虚血瘀证的临床研究

目的观察通脉口服液对慢性肾炎气虚血瘀证患者血清肾纤三项及症状、体征的影响。方法60例中医辨证为气虚血瘀的慢性肾炎患者随机分为治疗组和对照组各30例。治疗组采用常规西医降压、抗凝、降脂等对症治疗；治疗组在常规西医治疗的基础上加用通脉口服液，治疗3个月后测定两组患者血清肾纤三项指标，观察其临床症状的改善程度。结果两组均可在一定程度上降低肾纤三项的水平，改善临床症状和体征。两组相比较，治疗组作用更为明显。且差异有显著性（P〈0．05）。结论通脉口服液可有效地减轻慢性肾炎气虚血瘀证患者肾纤维化的程度。     

单核四价锰配合物Mn^IV（salae）2的合成和晶体结构

目的合成高价锰配合物并研究其晶体结构。方法席夫碱配体H2salae(水杨醛缩乙醇胺)与Mn(OAc)3.2H2O在甲醇-水混合溶剂中反应,溶液静置,溶剂挥发后得到单晶。结果配合物MnIV(salae)2的单晶属六方晶系,空间群R-3,晶胞参数:a=1.7907(1)nm,b=1.7907(1)nm,c=2.8162(3)nm,α=90°,β=90°,γ=120°,V=7.8201(1)nm3,Z=18,Mr=381.28,Dc=1.457 g/cm3。结论MnIV由两个席夫碱配体进行配位,每个配体提供两个氧原子和一个氮原子。中心锰IV处于八面体的配位环境中。不同单元的分子由分子间C—H…O氢键和C—H…π弱相互作用连接在一起,形成三维结构。     

PVC膜铂(Ⅳ)离子选择性电极的研制

本文介绍了PVC膜铂离子选择性电极的研制过程及性能。电极响应曲线的线性范围为:3×10~(-3)～1×10~(-6)M,电极斜率为:29MV/△PC,适宜的PH范围是:3～6,电极的正向导电内阻为0.3MΩ,反向为无穷大。     

Sub-classes and evolution stability of Wolfram''s classesin the total-rule cellular automata

In this paper, we propose a concept of sub-classes and its evolution stability for the Wolfram's classes. Firstly, we obtain the sub-classes of the Wolfram's class IV, gene-piece of these sub-classes and their existing circumstance. Secondly, we introduce a new concept, the evolution stability, for the Wolfram's classes and sub-classes of Wolfram's class IV. Lastly, we find that Wolfram's classes I, II, and III have the evolution stability, but sub-classes of the Wolfram's class IV have not the evolution stability for the total rule cellular automata.     

橙色粘球菌四型菌毛膜复合物的发现

细菌四型菌毛(T4P)不仅在生物膜形成、DNA吸收和侵染致病等生物过程中发挥着重要的作用,同样也能够介导固体界面的滑动运动。尽管T4P具有非常重要的作用,但橙色粘球菌T4P系统中蛋白间组装机制的研究仍处于初级阶段。T4P系统pil N/O/P/Q单基因的敲除与回补实验显示敲除菌株导致橙色粘球菌群体运动完全丧失,而pil N/O/P/Q相应基因回补则恢复突变株群体运动。Pil N/O/P/Q蛋白通过酵母双杂交(Y2H)检测蛋白间的相互作用发现,定位于周质空间的Pil P不仅与外膜蛋白Pil Q的C末端相互作用,也与内膜蛋白Pil O相互作用。遗传学与酵母双杂实验结果显示着Pil P蛋白是联通内外膜的桥梁,暗示着Pil N/O/P/Q蛋白形成跨越内膜、细胞周质和外膜的整合复合物结构,并在菌毛延伸和收缩过程中发挥着重要的作用,为阐明橙色粘球菌菌毛的延伸与收缩的分子机制奠定坚实基础。     

P2P平台利率影响因素分析

针对利率市场化进程不断推进,利率成为互联网金融监管中最重要的环节,提出P2P借贷平台利率影响因素模型,实证研究P2P借贷利率的影响因素,并对借款者违约意向做出深入研究,为P2P借贷平台的发展提供参考与理论依据;研究发现:订单信息、借款人财务信息、信用信息、羊群效应对借款利率都产生影响;违约次数能够体现出个人信用状况,但在探究利率定价机制中并不显著,违约次数主要由还款能力和还款意愿决定,投资者应当重视欠款金额和信用查询记录,避免借款者产生道德风险;投资者人数对借款利率产生负向影响,表明当前投标人数能够对获得后续投标的可能性产生积极影响,投资者对于利率较低的订单,认为借款人的信用状况良好。     

仙方玉容膏中黄芪甲苷含量的测定

目的:建立仙方玉容膏中黄芪甲苷含量的高效薄层色谱测定方法。方法:以氯仿-甲醇-水(13∶6∶2)为展开剂,扫描波长λS=530nm,λR=700nm。结果:线性范围0 25～4ug,回收率97 6～103 4%,RSD2 84%(n=5)。结论:本法简便、灵敏、重线性好,可用于测定仙方玉容膏中黄芪甲苷含量和质量标准的控制。